Light++microscopy++(Maggie+Brower)

The technique light microscopy because it is a technique that is used often even for students. The process involves using a microscope and studying cells of various organisms. It is very interesting because microscopes are used all the time in biology classes and having a personal experience using a microscope; it is very interesting to observe cells. Light microscope has improved a lot over the years. The improvement of computers and informatics has made it much easier to study cells (Antony et al., 2013). They added a diagram that they believe is the success to experiments using light microscopy.
 * Background:**

In figure 1 the diagram basically explains that if the raw data has good quality and the microscope is at the right power, good results will be produced. Also there has been new used for the traditional microscope. Three noble prize winners, Eric Betzig, Stefan Hell, and William Moerner, came up with the technique called stimulated emission depletion microscopy. This technique was developed in 2000 but they won the noble price in 2014. This technique involves using florescence in the microscope which emits light to give more detail in the cell structures (Clery, 2013). This changes research for many people because it will be able to improve the quality of the results when using a microscope.  In figure 2 light microscopy can be used to study oocyte which is composed of microtubules. Oocyte is important during meiosis and chromosome alignment (Cammano et al., 2011). These researchers used polarized light microscopy to study these cells and that was beneficial because no staining was required. The cells can be observed at its original state. The picture below shows an example of this.
 * New Research**

Bibliography:

 Antony, P.M.A., Trefois, C., Stojanovic, A., Baumuratov, A.S., & Koxak, K. 2013. Light microscopy applications in systems biology: opportunities and challenges. //Cell Communication and Signaling,// 11:24

Cammano, J.N., Maside, C., Gil, M.A., Munoz, M., Cuello, C., Diez, C., Sanchez-Osorio, J.R., Martin, D., Gomis, J., Vazquez, J.M., Roca, J., Corrocera, S., Martinez, E.A., &Gomez, E. 2011. Use of polarized light microscopy in porcine reproductive technologies. //Theriogenology, 76:669-677//

Clery, Daniel. 2014. Barrier-breaking microscopy methods that revealed cell’s inner life win nobel. [|http://news.sciencemag.org/chemistry/2014/10/updated-barrier-breaking- microscopy-]methods-revealed-cells-inner-life-win-nobel