Fluorescent+staining

media type="youtube" key="i6jXWl3EUjA?feature=player_detailpage" height="360" width="640" __F____luorescent staining definition and Description__ Fluorescent staining can be defined as a staining procedure in which a staining dye is used with the purpose to combine selectively with certain tissue components and that will then fluoresce upon irradiation with ultraviolet or violet-blue light.

__What can it be used for?__ It can be used to view samples more clearly, isolate certain components and differentiate certain parts of a cell that are being viewed on a lab slide. As a result, the materials being viewed will result in different types of fluorescent stains. This technique allows researchers to view bacteria, fungi, etc. separately by suing different fluorescent stains materials. In order to use this technique, first the sample has to be placed on a slide, the proper fluorescent stain is then added with a dropper to the slide and lastly, the fluorescent stained slide can be viewed under a microscope.

__Article #1__ One of the articles that I found describes the application of fluorescent stains to cells and food soil to optimize a dual staining method that can be used in the quantitative analysis of surface cleanability. The technique is important because other techniques used before took at least eighteen hours to obtain results. Moreover, they do not determine the presence of residual organic material on a surface, which may interfere with cleaning and disinfection (Whitehead et. al. 2009). This method was successful in demonstrating the hygienic status of a surface in an industrial setting. This method was also of “significant in assays to determine the effectiveness of cleaning and disinfecting regimes on the retention of cells and organic soil” (Whitehead et. al. 2009).

__Article #2__ There is lack of techniques on how to detect and quantitate viable virus directly in Culicoides sonorensis cells. The other article that I found “fluorescent staining techniques were developed to visualize and quantitate BTV infection in Culicoides cell culture by both an endpoint titration and an agarose overlay fluorescent focus assay” (Mecham et. al. 2009). The Techniques described in the article could be modified to accommodate a variety of viruses by substituting the appropriate primary monoclonal antibody. Furthermore, “The insect cell lines used in this study were derived from the vector of BTV, as well as related orbiviruses, therefore, the assays described have potential for numerous research and diagnostic applications” (Mecham et. al. 2009).

__Article #3__ In this article, “fluorescent succinimidyl benzazole derivatives were synthesised and successfully used to stain Candida albicans ATCC 10231 cells. The dyes were characterised by means of infrared, 13C and 1H NMR spectroscopies and elemental analysis” (Santos et. al. 2011). As a result, the new probes are fluorescent in the yellow-green region by an intramolecular proton transfer mechanism with a large Stokes shift. In addition, the present dyes were used as new probes to study the micromorphology of Candida albicans.

Works Cited: Mecham, J. O., Brown, P. L., & McHolland, L. E. (2009, June). In situ immune infrared fluorescent staining for detection andquantitation of bluetongue virus in Culicoides insect cell culture. Journal of Virological Methods, 158(1-2), 110-113.

Santos, R., Faleiro, N., Campo, L., Scroferneker, M., Corbellini, V., Rodembusc, F., & Stefani, V. (2011, June 8). Synthesis and photophysical properties of novel succinimidyl benzazole derivatives, evaluated by Candida albicans ATCC 10231 fluorescent staining. Tetrahedron Letters, 52(23), 3048-3053.

Whitehead, K. A., Benson, P., & Verran, J. (2009, September 30). Differential fluorescent staining of Listeria monocytogenes and a whey food soil for quantitative analysis of surface hygiene. International Journal of Food Microbiology, 135(1), 75-80.

[]